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For cDNA library construction and cloning applications, AsthaGen offers
high efficiency chemical competent cells. The cells are designed to obtain transformation
efficiencies exceeding >1-5x 108 cfu /µg supercoiled pUC19 DNA. These cells are ideal for cloning
and propagation of plasmid, cosmid, or fosmid clones. The strains allows methylated genomic DNA
isolated directly from mammalian or plant cells to be cloned without deletions or re-arrangements.
They give high yield and high quality plasmid DNA due to the endA1 mutation. E. coli SJ T1 cells
contains tonA gene that confers resistance to bacteriophages T1 and T5.
Advantages of AsthaGen’s Electro Competent Cells:
- High transformation efficiency – 1-5 x 108 cfu /µg supercoiled pUC19 DNA for competent cells
- Allows blue/white screening through ß-galactosidase complementation
- E. coli T1 cells confers resistance to bacteriophages T1 and T5
- Available as ready-to-use aliquot either in tubes or in 96 well format
Components
- Competent Cells
- pUC19 DNA
- Instruction Manual
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