• Cloning Kits and Reagents
• Electrophoresis Kits and Reagents
• Microarray Related Kits
• DNA/ RNA Isolation Kits
• cDNA Synthesis Kits
• DNA Amplification Kits
• Tissue ReadyLysates™
• Pre-Made Western Blots
• Immuno Detection Kits
• Enzymes
• Pure™ Reagents
• Essential Lab Supplies
• DNA Purification columns
• Antibodies
• Recombinant Proteins

• Plasmid DNA Manufacturing
• DNA/ RNA Isolation
• cDNA Library Construction
• Genomic Library Construction
• Genomic Library Screening Services
• Real Time PCR
• DNA Sequencing
• Oligo Synthesis
• Gene Cloning and Expression
• Recombinant Protein Purification

Achieve Ligation within 30 Minutes

• Ligation of cohesive or blunt ended DNA is completed within 30 minutes
• All necessary reagents including competent cells are provided with the kit

AsthaGen’s Quick DNA Ligation System provides reagents for ligation within 30 minutes. The reagents provide the researcher a quick, reliable and convenient system for shotgun cloning of DNA fragments or PCR products into plasmid, cosmid or lambda phage vectors.

• Ligation completed within 30 minutes
• All necessary reagents including competent cells are provided with the kit

Applications

• Rapid cloning of DNA for gene expression purposes
• Construction of recombinant plasmid for DNA sequencing

Kit Components

• Quick DNA Ligase, 500 units
• 10x Ligase Buffer, 100 µl
• 10 mM dATP, 25 µl
• Nuclease Free Water, 500 µl
• E. coli Competent Cells, 10x 50 µl
• Instruction Manual

Storage Condition and Stability

Kit components are stored at -20o C and -80o C and stable for 3-6 month.

Ordering Information

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