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For cDNA library construction and cloning applications, AsthaGen offers
high efficiency electro competent cells. The cells are designed to obtain transformation efficiencies
exceeding 1-5x1010 cfu /µg supercoiled pUC19 DNA. These cells are ideal for cloning and propagation of
plasmid, cosmid, or fosmid clones. The strains allows methylated genomic DNA isolated directly from
mammalian or plant cells to be cloned without deletions or re-arrangements. They give high yield and
high quality plasmid DNA due to the endA1 mutation. E. coli SJ T1 cells contains tonA gene that confers
resistance to bacteriophages T1 and T5.
Advantages of AsthaGen’s Electro Competent Cells:
- High transformation efficiency – 1-5 x 1010 cfu /µg supercoiled pUC19 DNA for electro competent cells
- Allows blue/white screening through ß-galactosidase complementation
- E. coli T1 cells confers resistance to bacteriophages T1 and T5
- Available as ready-to-use aliquot either in tubes or in 96 well format
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