• Cloning Kits and Reagents
• Electrophoresis Kits and Reagents
• Microarray Related Kits
• DNA/ RNA Isolation Kits
• cDNA Synthesis Kits
• DNA Amplification Kits
• Tissue ReadyLysates™
• Pre-Made Western Blots
• Immuno Detection Kits
• Enzymes
• Pure™ Reagents
• Essential Lab Supplies
• DNA Purification columns
• Antibodies
• Recombinant Proteins

• Plasmid DNA Manufacturing
• DNA/ RNA Isolation
• cDNA Library Construction
• Genomic Library Construction
• Genomic Library Screening Services
• Real Time PCR
• DNA Sequencing
• Oligo Synthesis
• Gene Cloning and Expression
• Recombinant Protein Purification

Reagents for Subtracting Differentially Expressed mRNA for Microarray and cDNA Library Construction Purposes

AsthaGen’s mRNA Subtraction Kit is designed for identifying differentially expressed genes for microarray and cDNA library construction purposes. In higher eukaryotes, biological processes such as cellular growth and organogenesis are mediated by differential gene expression. To understand molecular regulation of these processes, differentially expressed genes of interest must be identified for microarray application and cDNA clone identification purposes.

Kit Components

• Oligo dT/ Random Primer Mix
• Reverse Transcriptase
• RNAse Inhibitor
• RNase H
• 10x RT Buffer
• dNTP Mix
• mRNA-cDNA Subtraction Buffer
• Oligo-dT Cellulose Column
• 10x Wash Buffer
• mRNA Elution Buffer
• 3M LiCl Solution
• Glycogen

Storage Condition and Stability

mRNA Subtraction System is stored at 4o C and stable for six months.