• Cloning Kits and Reagents
• Electrophoresis Kits and Reagents
• Microarray Related Kits
• DNA/ RNA Isolation Kits
• cDNA Synthesis Kits
• DNA Amplification Kits
• Tissue ReadyLysates™
• Pre-Made Western Blots
• Immuno Detection Kits
• Enzymes
• Pure™ Reagents
• Essential Lab Supplies
• DNA Purification columns
• Antibodies
• Recombinant Proteins

• Plasmid DNA Manufacturing
• DNA/ RNA Isolation
• cDNA Library Construction
• Genomic Library Construction
• Genomic Library Screening Services
• Real Time PCR
• DNA Sequencing
• Oligo Synthesis
• Gene Cloning and Expression
• Recombinant Protein Purification

Reagents for Isolating mRNA from Total RNA

• High quality mRNA is isolated within one hour
• Quick and easy protocol
• High yield of mRNA with consistent results
• All necessary reagents are provided with kit

AsthaGen’s mRNA Isolation System provides reagents for isolating mRNA from total RNA in less than one hour. Total RNA is mixed with oligo dT cellulose. Poly A+ mRNA is annealed with oligo dT cellulose and captured. Unbound tRNA, ribosomal RNA, DNA are removed by washing column with 1x wash buffer. mRNA is eluted from oligo dT cellulose and ready for usage in either cDNA synthesis for library construction or probe labeling for microarray analysis.

Kit Components

• Oligo dT Cellulose Mini Columns – 15 Columns
• 1xAnnealing Buffer – 25 ml
• 1x Wash Buffer – 25 ml
• 1x Elution Buffer - 10 ml
• Instruction Manual

Storage Condition and Stability

mRNA Isolation System is stored at 4o C and stable for six months.