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AsthaGen offers customized gene cloning services for expression in a variety
of vector systems.
Choice of Prokaryotic, Eukaryotic and Baculovirus Expression Vectors
The advantages of AsthaGen’s gene cloning and expression services are:
- Competitive strategies and price to fit your budget
- Compatibility – prokaryotic, eukaryotic or baculovirus expression systems
- Experienced technical staff with more than 10 years of experience
- High throughput and fast turnaround time
Service Overview
1. DNA Cloning
- Isolating the gene of interest from a supplied vector and sub cloning it into a plasmid or
baculovirus transfer vector
- Verification of recombinant plasmid or baculovirus transfer vector by restriction mapping and
DNA sequencing
2. Gene Expression
- Preparation and purification of recombinant vector suitable for transfection purposes
- Transfection of cDNA into mammalian/insect/bacterial cell
- Selection of over expression cells under optimized conditions
- Western blot analysis to test the expression
3. Scale up and Optimization
- Large scale growth of over-expression cells
- Preparation and purification of recombinant proteins and QC by Western Blot
Benefits of Expression in Mammalian and Insect Cells
- Eukaryotic post-translational modification
- Proper protein folding and function
- High expression levels
- Easy scale up and protein purification
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