• Cloning Kits and Reagents
• Electrophoresis Kits and Reagents
• Microarray Related Kits
• DNA / RNA Isolation Kits
• cDNA Synthesis Kits
• DNA Amplification Kits
• Tissue ReadyLysates™
• Pre-Made Western Blots
• Immuno Detection Kits
• Enzymes
• Pure™ Reagents
• Essential Lab Supplies
• DNA Purification Columns
• Antibodies
• Recombinant Proteins

• Plasmid DNA Manufacturing
• DNA/ RNA Isolation
• cDNA Library Construction
• Genomic Library Construction
• Genomic Library Screening Services
• Real Time PCR
• DNA Sequencing
• Oligo Synthesis
• Gene Cloning and Expression
• Recombinant Protein Purification

AsthaGen offers high-quality customized genomic libraries for the specific needs of fellow researchers. Our staff has over ten years of experience in constructing genomic libraries from variety of cells and tissues such as:

Human, Mouse, Rat, Monkey, Pig, Plant, Algae, Bacteria, Fungi, Yeast, Fruit Fly, Protozoa, Fish, etc.

AsthaGen offers the following vectors for genomic library construction purposes:

• Shotgun Genomic Library in Plasmid Vectors
• Genomic Library in Lambda Vectors
• Genomic Library in Cosmid Vectors

• Choice of vector for library construction (plasmid, cosmid or lambda phage vectors)
• Low vector background and high percentage of recombinants (over 90% recombinant)
• Large number of primary clones (2-3 x 10⁷)
• Test for T1 phage contamination
• Rigorous QC steps to test RNA or host DNA contaminations
• Size-fractionated large insert genomic library:
  • Average insert sizes: 1-2 kb or 2-4 kb – plasmid library
  • Average insert sizes: 15 kb – lambda library
  • Average insert size: 30 kb – cosmid library