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AsthaGen offers Recombinant Protein Purification Services for cDNA
cloned into bacterial, mammalian or insect cells. Recombinant Protein Production is a core
activity of groups involved in high throughput screening functional proteins for validation
and structural biology. Our Recombinant Protein Purification Services is a complete solution
for your customized needs that partners with your research team working side by side.
Service Overview
1. Assessment and Optimization of Expression
- Expression of proteins are optimized by testing various cloning vectors and host cells
- Time course study of expression to minimize protein degradation and proper folding
- Small scale protein purification and QC by SDS-PAGE and western blot analysis
2. Protein Purification
- Large Scale growth of over expressing construct and cells
- Harvesting cells and cell lysis by sonication
- Protein purification on affinity tag resins
- Secondary purification on anion exchange resin or by gel filtration
- Dialysis and western blot analysis
- Delivery of proteins
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