• Cloning Kits and Reagents
• Electrophoresis Kits and Reagents
• Microarray Related Kits
• DNA / RNA Isolation Kits
• cDNA Synthesis Kits
• DNA Amplification Kits
• Tissue ReadyLysates™
• Pre-Made Western Blots
• Immuno Detection Kits
• Enzymes
• Pure™ Reagents
• Essential Lab Supplies
• DNA Purification Columns
• Antibodies
• Recombinant Proteins

• Plasmid DNA Manufacturing
• DNA/ RNA Isolation
• cDNA Library Construction
• Genomic Library Construction
• Genomic Library Screening Services
• Real Time PCR
• DNA Sequencing
• Oligo Synthesis
• Gene Cloning and Expression
• Recombinant Protein Purification

AsthaGen offers high-quality customized cDNA libraries for the specific needs of fellow researchers. Our staff has over ten years of experience in constructing cDNA and genomic libraries from a variety of cells and tissues such as:

Human, Mouse, Rat, Monkey, Plant, Algae, Bacteria, Fungi, Yeast, Fruit Fly, Protozoa, Fish, etc.

• Standard cDNA Libraries
• Normalized cDNA Libraries
• Subtracted cDNA Libraries

Standard cDNA Library Construction

• We guarantee to provide 3-5x10⁶ primary clones
• Average insert size is least 1-1.5 kb and Over 90% recombinants
• The libraries can be made directionally and in the choice of vector for your special needs
• Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
• For quality control purposes we test 10-15 random clones by PCR or restriction digestion
• The library is provided in glycerol or DMSO

Normalized cDNA Libraries

• Normalization is carried out at various Cot levels (Cot₁₀, Cot₅₀ or Cot₅₀₀) according to relative abundance to genes that needs to be normalized
• Percent Normalization is tested by:
  • Relative PCR for house keeping genes and low abundant genes
  • Colony/plaque hybridization of 3,000 – 30,000 clones before and after normalization
  • DNA sequencing of 96 random cDNA clones
• We guarantee to provide 1x10⁶ primary clones
• Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
• Average inserts size of at least 1 -1.5 kb and over 90% recombinants
• The libraries can be made directionally and in the choice of vector for your specific needs

Subtracted cDNA Libraries

• Subtraction is carried out according to relative abundance to genes that needs to be subtracted
• Percent Subtraction are tested by:
  • Relative PCR for house keeping genes and low abundant genes
  • Colony/plaque hybridization of 3,000 – 30,000 clones before and after subtraction
  • DNA sequencing of 96 random cDNA clones
• We guarantee to provide 1x10⁶ primary clones
• Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
• Average inserts size of at least 1 -1.5 kb and over 90% recombinants
• The libraries can be made directionally and in the choice of vector for your specific needs